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The camera measures 4.5 x 2.8 x 1.63 (WHD in inches) and tips the scales at 15.8 ounces with the pancake lens and battery. We liked it and it’s a real standout, but check out the accompanying photos to see if this design appeals to you. With the optional 17mm f/2.8 pancake lens attached, it has a boxy, retro shape some might find cool while turning off others. Pick up the new PEN and it looks like a 35mm film camera from back in the day. Let’s see if Olympus has delivered on the dream and if you should consider it instead of a DSLR. One of the newest of these cameras is the Olympus PEN E-PL1 which uses the same 12MP chip found in the company’s much larger and heavier E-30 and E-620 DSLRs. Olympus and Panasonic have jumped into this arena and Sony is planning to join them later this year.
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Rather than beating their heads against the wall by competing directly against them, other manufacturers are zeroing in a new type of camera-one with a large imaging sensor, smaller size and interchangeable lenses so you can get the photographic benefits of a DSLR without breaking your shoulder carrying it around all day. It’s hardly a secret that Canon and Nikon dominate the DSLR market. This assay can quickly and accurately quantify the number of human astrocytes and microglia in mixed cell culture and can be applied to quantifying a range of other cells/objects with complex morphology in neuroscience research.
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Significant correlations have been consistently achieved between the data obtained from CCC analysis and manual cell counts. The time needed to analyze each image is 1.82 s. The CCC assay consists of four main aspects: image processing with a unique combination of morphology filters digital thresholding integrated morphometry analysis and a configuration of object standards. This method, called the complex cell count (CCC) assay, utilizes a combination of image processing and analysis operations from MetaMorph™ (Version 6.2.6, Molecular Devices). We have developed a fully automated, fast, accurate and objective method for the quantification of primary human GFAP-positive astrocytes and CD45-positive microglia from images of mixed cell populations. However, it has been more difficult to automate the counting of specific cell types with complex morphologies in mixed cell cultures. The ability to diminish apoptosis may contribute to maintaining healthy tissues.Īutomated image-based and biochemical assays have greatly increased throughput for quantifying cell numbers in in vitro studies. Importantly, both cerium oxide and platinum nanoparticles reduce oxidant-mediated apoptosis in target cells as judged by the activation of caspase 3. Furthermore, cerium oxide and platinum nanoparticles demonstrated superoxide dismutase catalytic activity, but did not promote hemolytic or cytolytic pathways in living cells. Using surface plasmon-enhanced microscopy, we find that these nanoparticles associate with cells. Cerium oxide and platinum nanoparticles were found to be 16 ± 2.4 and 1.9 ± 0.2 nm in diameter, respectively. In the present study, we directly compare these two classes of catalytic nanoparticles. Cerium oxide and platinum nanoparticles represent two potentially therapeutic nanoparticles that de-toxify ROS. Several diseases, including many blinding eye diseases, are promoted by excessive oxidant stress due to reactive oxygen species (ROS). High-Content Screening with AgileOptix TechnologyĬatalytic nanoparticles represent a potential clinical approach to replace or correct aberrant enzymatic activities in patients.PROTEIN DETECTION, QUANTITATION, ANALYSIS.NUCLEIC ACID (DNA/RNA) DETECTION & ANALYSIS.SpectraTest Validation Plates and Recertification.GXP SOFTWARE INSTALLATION AND VALIDATION.
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